According to Official Guinness Records,
The oldest human DNA found and analysed ("sequenced") was taken from the thighbone of a 400,000-year old human-like species whose remains were found in a cave called “Sima de los Huesos” (“Pit of Bones”), in the province of Burgo, northern Spain. The results of the study by scientists at the Max Planck Institute for Evolutionary Anthropology in Leipzig, Germany, with co-authors in Spain and China, were published in the journal Nature on 4 December 2013. The study suggests that we may share a common human ancestor with the Denisova hominins, a similar, yet separate species to the Neanderthals, who also share a common ancestor with modern man. The fact that this connection was found in bones in Spain came as a surprise, as until then scientists thought that the Denisovans lived in Eastern Asia, in present-day Siberia, and not in Western Europe.
For a complete list of 2013 records, please visit 2013 Guinness Records in Spain.