According to Official Guinness Records,
The largest parade of Segways consists of 157 participants and was achieved by Metafinanz Informationssysteme GmbH (Germany), in Absberg, Germany, on 29 July 2016. This record was achieved as part of a team building event called "One team - one record".
For a complete list of 2016 records, please visit 2016 Guinness Records in Germany.