According to Official Guinness Records,
The largest laundry lesson involved 318 people, achieved by Lion NANOX Team (Japan) at Lion Hirai Research Laboratory, in Edogawa, Tokyo, Japan, on 29 November 2017.The participants learned the basic chemistry behind how laundry detergents works, in addition to actually trying their hand at cleaning a dirty sock.
For a complete list of 2017 records, please visit 2017 Guinness Records in Japan.