According to Official Guinness Records,
The most people blowing train whistles simultaneously is 5,527, and was achieved by Boiler Gold Rush (USA) at Purdue University in West Lafayette, Indiana, USA, on 15 August 2018.The attempt was part of the Purdue University freshman orientation week programing, and commemorates the school's mascot, the Boilermaker Special.
For a complete list of 2018 records, please visit 2018 Guinness Records in United States.