According to Official Guinness Records,
The most aces served by an individual player in a Wimbledon tennis match is 113 by John Isner (USA) against Nicolas Mahut (France) at the 2010 Wimbledon Championships, in Wimbledon, London, UK, from 22-24 June 2010.
For a complete list of 2010 records, please visit 2010 Guinness Records in United Kingdom.